Fig. 6

miR-124-3p downregulated E2F3 expressions by direct interactions in H9C2 cells. A Bioinformatics for direct interaction between E2F3 and miR-124-3p. B Relative luciferase activity for cells transfected by E2F3 WT, E2F3 MUT with miR-NC or miR-124-3p mimic. C E2F3 mRNA expression in H9C2 cells transfected by miR-NC or miR-124-3p mimic. D E2F3 protein expression in H9C2 cells transfected by miR-NC or miR-124-3p mimic. E E2F3 protein expression in H9C2 cellstransfected by si-NC, si-MAPKAPK5-AS1, Vector, or pc-MAPKAPK5-AS1. F E2F3 protein expression in cardiac tissue injected by vehicle, LPS, or LPS + si-MAPKAPK5-AS1. G E2F3 protein expression in cardiac tissue treated by vehicle, LPS (5 mg/kg), or LPS (10 mg/kg). Each column represents the mean ± SEM. Statistical differences were evaluated with Student’s t-test or One-way ANOVA followed by Tukey’s post-hoc test. *P < 0.05, **P < 0.01, n = 3