Fig. 2
From: The collagen structure of C1q induces wound healing by engaging discoidin domain receptor 2
Co-localization of C1q to DDR2-expressing cells. A DDR2 expression in the HT1080 cell line was determined using confocal microscopic images and flow cytometric analyses. B Confocal microscopy analysis showing co-localization of C1q with DDR2 in HT1080 cells. The cells were treated with 2 μg/mL C1q in the absence or presence of 2 μg/mL sDDR2. Confocal images of the cells show dual staining for DDR2 (red) and C1q (green). The phenomenon was attenuated with the addition of sDDR2. C Proximity ligation assay (PLA) of DDR2 and C1q. The graph shows the mean ± SEM of three independent experiments. *** p < 0.001, **** p < 0.0001 (one-way ANOVA)