Fig. 3
From: The collagen structure of C1q induces wound healing by engaging discoidin domain receptor 2
C1q-DDR2 binding promotes wound healing. A DDR2 phosphorylation was evaluated by immunoprecipitation with rabbit anti-DDR2 Ab and western blot with anti-phosphotyrosine Ab (4G10) or mouse anti-DDR2 Abs. HT1080 cells were incubated with the indicated concentration of collagen-I or C1q for 24 h. One representative image of three independent experiments. B HT1080 cells were grown to confluence in 12-well plates; a straight scratch was made and then treated with 20 μg/mL C1q for the indicated time. The wound areas were calculated and expressed as the percentage of wound closure to the initial wound areas. Values are presented as the means ± SEM of three independent experiments. C Western blot of DDR2 knockdown in HT1080 cells. D The wound healing assay was performed as described in 3B after transfection of DDR2 shRNA or scrambled control RNA (shSCR). Values are presented as the means ± SEM of three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001 (one-way ANOVA)