Fig. 4

LncRNA SNHG1 overexpression promoted osteoclast differentiation by targeting miR-181c-5p. The BMSCs were transfected by vector-NC, SNHG1 vector, SNHG1 vector with miR-NC, or SNHG1 vector with miR-181c-5p mimics, and induced with M-CSF and RANKL for 10 days. A Representative images of TRAP staining in four groups. B–D After induction, the mRNA and protein expression of NFATc1, RANKL and CTSK in BMSCs were measured through qPCR and western blot. E and F Cell migration of treated BMSCs was determined using Transwell assay. G–H Adhered cells were stained with crystal violet and cell number was counted. *P < 0.05, **P < 0.01, ***P < 0.001