Fig. 6

LncRNA SNHG1 regulated SFRP1/Wnt signaling pathway by interacting with miR-181c-5p. A StarBase analysis showed the predicted miR-181c-5p binding sites in the 3′ UTR of SFRP1 mRNA. B Dual luciferase reporter assay performed to verify the association of miR-181c-5p and SFRP1. C The BMSCs were transfected with miR-181c-5p mimics or miR-NC for 48 h, the expression level of SFRP1 was detected by qPCR. D–F The BMSCs were transfected with miR-NC, miR-181c-5p mimics, miR-181c-5p mimics with vector NC, miR-181c-5p mimics with SFRP1vector for 48 h. The mRNA level of SFRP1 was detected by qPCR (D), the protein levels of SFRP1, Wnt3a and β-catenin were detected with western blot (E), the cytoplasmic and nuclear expression of β-catenin were observed by immunofluorescence (F). G and H After transfection with vector-NC, SNHG1 vector, SNHG1 vector with miR-NC, SNHG1 vector with miR-181c-5p mimics for 48 h, the mRNA and protein expression of SFRP1, Wnt3a and β-catenin in BMSCs were assessed through qPCR and western blot, respectively. *P < 0.05, **P < 0.01, ***P < 0.001