Fig. 7

LncRNA SNHG1 silence alleviated the symptoms of osteoporosis in OVX mice. Mice were randomly divided into four groups: Sham, OVX, OVX + sh-SNHG1 and OVX + sh-SNHG1 + miR-181c-5p inhibitor groups. Mice were injected the above and femurs were removed after 8 weeks. A The SNHG1 and miR-181c-5p expression levels in femoral tissue were detected through qPCR. B Representative HE staining of distal femoral sections showing the bone formation. C Representative images of Masson staining in femurs showing the new bone growth. D Representative photomicrographs of TRAP staining in distal femora showing the bone destruction. E NFATc1 expression was assessed by performing IHC staining. F The protein expression of ALP, OCN, RUNX2, VEGF, NFATc1, RANKL and CTSK were measured using western blot. *P < 0.05, **P < 0.01, ***P < 0.001