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Fig. 2 | Molecular Medicine

Fig. 2

From: IκB kinase promotes Nrf2 ubiquitination and degradation by phosphorylating cylindromatosis, aggravating oxidative stress injury in obesity-related nephropathy

Fig. 2

Oxidative stress injury was observed in kidney tissues of ORN model mice. A The production of ROS in the kidneys of ORN model mice and control mice was measured using DHE staining. B–E MAD level, SOD activity, CAT activity and GSH-Px activity were measured in mouse kidneys. F Representative IHC staining of Nrf2 and HO-1 in mouse kidneys. G Western blot analysis of p-IKK, IKK, p-CYLD, CYLD, Nrf2 and HO-1 expression in mouse kidneys. ORN, obesity-related nephropathy; ROS, reactive oxygen species; DHE, dihydroethidium; MAD, malondialdehyde; SOD, superoxide dismutase; CAT, catalase; GSH-Px, glutathione peroxidase; IHC, immunohistochemistry; Nrf2, NF-E2-related factor 2; HO-1, haem oxygenase 1; IKK, IκB kinase; CYLD, cylindromatosis. Significance was assessed by a Student's t test. The experimental data are shown as the mean ± SD. N = 10. *P < 0.05, **P < 0.01, ***P < 0.001

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Molecular Medicine

ISSN: 1528-3658