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Fig. 5 | Molecular Medicine

Fig. 5

From: IκB kinase promotes Nrf2 ubiquitination and degradation by phosphorylating cylindromatosis, aggravating oxidative stress injury in obesity-related nephropathy

Fig. 5

Knockdown of CYLD attenuated the effect of an IKK inhibitor on oxidative stress injury in an in vitro ORN model. Cells were transfected with sh-CYLD to silence CYLD expression and divided into four groups: ox-LDL + sh-NC, ox-LDL + TPCA1 + sh-NC, ox-LDL + sh-CYLD, and ox-LDL + TPCA1 + sh-CYLD. A and B The knockdown efficiency of sh-CYLD was assessed using qRT–PCR and western blotting. C Effect of CYLD knockdown on TG levels in treated HK-2 cells. D Lipid deposition in different groups was measured using Oil Red O staining. E and F MAD level and SOD activity of treated HK-2 cells. G and H ROS levels and ΔΨm of treated HK-2 cells were measured by DHE staining and JC-1 assay, respectively. CYLD, cylindromatosis; IKK, IκB kinase; ORN, obesity-related nephropathy; ox-LDL, oxidized low-density lipoprotein; NC, normal control; TG, triglyceride; HK-2, human kidney 2; MAD, malondialdehyde; SOD, superoxide dismutase; DHE, dihydroethidium; ROS, reactive oxygen species; ΔΨm, mitochondrial membrane potential. Significance was assessed by an ANOVA test. The experimental data are shown as the mean ± SD. N = 3. *P < 0.05, **P < 0.01, ***P < 0.001

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