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Fig. 6 | Molecular Medicine

Fig. 6

From: IκB kinase promotes Nrf2 ubiquitination and degradation by phosphorylating cylindromatosis, aggravating oxidative stress injury in obesity-related nephropathy

Fig. 6

The deubiquitinase CYLD reduced the ubiquitination of Nrf2. A The protein expression levels of p-Nrf2, Nrf2, HO-1 and NQO1 in treated HK-2 cells. B The interaction between CYLD and Nrf2 was analysed using Co-IP. C and D Co-IP was performed to assess the effect of CYLD on the ubiquitination of Nrf2. CYLD, cylindromatosis; Nrf2, NF-E2-related factor 2; HO-1, haem oxygenase 1; NQO1, NAD(P)H dehydrogenase quinone 1; HK-2, human kidney 2; Co-IP, co-immunoprecipitation. Significance was assessed by an ANOVA test. The experimental data are shown as the mean ± SD. N = 3. *P < 0.05, **P < 0.01, ***P < 0.001

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Molecular Medicine

ISSN: 1528-3658