Fig. 7
MG132 inhibited the promoting effect of CYLD knockdown on oxidative stress in ORN cells. The proteasome inhibitor MG132 was used to regulate the expression of Nrf2. Cells were divided into five groups: ox-LDL + sh-NC, ox-LDL + sh-CYLD, ox-LDL + MG132, ox-LDL + sh-CYLD + MG132 and ox-LDL + tempol. A–F The effect of MG132 on TG levels, lipid deposition, MDA levels, SOD activity, ROS levels and ΔΨm. G The protein expression of HO-1 and NQO1 was measured using western blotting. CYLD, cylindromatosis; ORN, obesity-related nephropathy; Nrf2, NF-E2-related factor 2; ox-LDL, oxidized low-density lipoprotein; NC, normal control; MAD, malondialdehyde; SOD, superoxide dismutase; DHE, dihydroethidium; ROS, reactive oxygen species; ΔΨm, mitochondrial membrane potential; HO-1, haem oxygenase 1; NQO1, NAD(P)H dehydrogenase quinone 1. Significance was assessed by an ANOVA test. The experimental data are shown as the mean ± SD. N = 3. *P < 0.05, **P < 0.01, ***P < 0.001