Fig. 1

The expression levels of lncRNA-ATB and TGF-β1 in cell models. a–c LncRNA-ATB expression levels in HK-2 cells exposed to different-concentration COM for 48 h (a), to 200ug/ml COM for different hours (b), and to 10 ng/ml TGF-β1 for different hours c by qRT-PCR. d, e TGF-β1 expression levels in HK-2 cells exposed to 200ug/ml COM for different hours by qRT-PCR d and by Western bolting (e), f Quantitative results of TGF-β1 protein levels. g The Smad3 phosphorylation levels were detected by Western bolting. h Quantitative results of p-Smad3 or Smad3 expression levels. i LncRNA-ATB expression levels in HK-2 cells after the inhibition of the TGF-β1 receptor by LY2109761 All above the experiments were repeated 3 times, values are mean ± SD. *P < 0.05, **P < 0.01 VS. 0ug/ml or 0H or control group, ^▲▲P < 0.01 vs. COM group, ^##P < 0.01 VS. TGF-β1 group