Fig. 2

Significant EMT changes of HK-2 cells exposed to COM or TGF-β1. a Morphological Changes of HK-2 cells exposed to 200ug/ml COM or 10 ng/ml TGF-β1 for 48 h under light microscope. b, c The mRNA b or protein c levels of EMT markers in HK-2 cells exposed to 200ug/ml COM or 10 ng/ml TGF-β1 for 48 h. d Quantitative results of EMT marker-protein levels. e The expression levels of E-cadherin and α-SMA were detected by immunofluorescence. All above the experiments were repeated 3 times, values are mean ± SD. *P < 0.05, **P < 0.01 VS. Control group. Scale bars = 200 μm