Fig. 3

Functional effects of lncRNA-ATB knockdown in HK‐2 cells exposed to COM. a The interference efficiencies of interfering lentivirus were validated by qRT‐PCR. b Cell damage was assessed by a Cytotoxicity LDH Assay Kit. c, d The expression levels of KIM-1 were detected by western blot analysis. e The expression levels of KIM-1 were detected by immunofluorescence. f Cell proliferation was assayed by CCK-8. g–k Cell apoptosis was analysed by flow cytometry g, h and TUNEL staining (i, j). All above the experiments were repeated 3 times, values are mean ± SD; *P < 0.05, **P < 0.01 VS. sh-NC group; ^##P < 0.01 VS. sh-NC + COM group. Scale bars = 200 μm