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Fig. 6 | Molecular Medicine

Fig. 6

From: LncRNA-ATB participates in the regulation of calcium oxalate crystal-induced renal injury by sponging the miR-200 family

Fig. 6

LncRNA-ATB regulates the COM-induced renal injuries via miR-200a. a, b The transfection efficiencies of miR-200a mimics (a) and inhibitors (b) were validated by qRT‐PCR. c The cell damage was assessed by a Cytotoxicity LDH Assay Kit. d, e The expression levels of KIM-1 were detected by western blot analysis. f The expression levels of KIM-1 were detected by immunofluorescence. g Cell proliferation was assayed by CCK-8. hk Cell apoptosis measured by flow cytometry h, i and TUNEL staining (j, k). All above the experiments were repeated 3 times, values are mean ± SD; *P < 0.05, **P < 0.01 vs. sh-NC + mimic-NC group; ##P < 0.01 vs. sh-ATB1 + inhibitor-NC group, Scale bars = 200 μm

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