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Fig. 2 | Molecular Medicine

Fig. 2

From: ADSCs enhance VEGFR3-mediated lymphangiogenesis via METTL3-mediated VEGF-C m6A modification to improve wound healing of diabetic foot ulcers

Fig. 2

ADSCs accelerate LEC proliferation, migration and lymphangiogenesis via the METTL3 pathway. sh-METTL3 and pcDNA-METTL3 plasmids were transfected into ADSCs for METTL3 knockdown and overexpression, respectively. sh-NC served as the negative control for sh-METTL3 plasmids, and pcDNA-empty vector served as the negative control for pcDNA-METTL3 plasmids. Subsequently, LECs were stimulated with ADSC-CM for 24 h. A RT-qPCR was performed to assess the levels of METTL3. B CCK-8 was conducted to assess LEC viability. C Transwell assays were carried out to evaluate LEC migration. D A tubule formation assay was performed to assess the tubule formation ability of LECs. Data represent the mean of three independent experiments. Error bars represent SD. *P < 0.05, **P < 0.01 or ***P < 0.001

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