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Fig. 4 | Molecular Medicine

Fig. 4

From: ADSCs enhance VEGFR3-mediated lymphangiogenesis via METTL3-mediated VEGF-C m6A modification to improve wound healing of diabetic foot ulcers

Fig. 4

ADSCs regulate VEGF-C-mediated lymphangiogenesis via the METTL3/IGF2BP2-m6A pathway. sh-METTL3 or sh-IGF2BP2 and pcDNA-VEGF-C were cotransfected into ADSCs. sh-NC served as the negative control for sh-METTL3 or sh-IGF2BP2, and the pcDNA-empty vector served as the negative control for pcDNA-VEGF-C plasmids. Subsequently, transfected ADSCs were cocultured with LECs. A RT-qPCR was performed to assess the levels of VEGF-C. B An ELISA was conducted to assess the levels of VEGF-C. C Transwell assays were carried out to evaluate LEC migration. D A tubule formation assay was performed to assess the tubule formation ability of LECs. Data represent the mean of three independent experiments. Error bars represent SD. *P < 0.05, **P < 0.01 or ***P < 0.001

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