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Fig. 2 | Molecular Medicine

Fig. 2

From: PIAS3 suppresses damage in an Alzheimer’s disease cell model by inducing the STAT3-associated STAT3/Nestin/Nrf2/HO-1 pathway

Fig. 2

Effects of PIAS3 overexpression on the survival of Aβ-treated SH-SY5Y cells. Cells were subjected to transfection with a PIAS3 overexpression vector or an empty vector for 1 d, and subsequently subjected to Aβ treatment (20 μM in 0.1% DMSO) for an additional 1.5 d. A, B qPCR and WB were performed to determine PIAS3 expression. C The CCK-8 assay revealed that effects of PIAS3 on cell survival 2 d after treatment. D CFA showed the influence of PIAS3 upregulation on cell growth. Data represent mean ± SEM. *p < 0.05, **p < 0.01. PIAS3 protein inhibitor of activated STAT3; qPCR quantitative polymerase chain reaction, Aβ amyloid beta, WB western blotting, CCK-8 Cell Counting Kit-8, DMSO dimethyl sulfoxide, CFA colony formation assay

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Molecular Medicine

ISSN: 1528-3658