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Fig. 3 | Molecular Medicine

Fig. 3

From: PIAS3 suppresses damage in an Alzheimer’s disease cell model by inducing the STAT3-associated STAT3/Nestin/Nrf2/HO-1 pathway

Fig. 3

Effects of PIAS3 overexpression on the apoptosis of Aβ-treated SH-SY5Y cells. Cells were subjected to transfection with a PIAS3 overexpression vector or an empty vector for 1 d, and subsequently subjected to Aβ treatment (20 μM in 0.1% DMSO) for an additional 1.5 d. AC qPCR and WB were used for determining Bcl-2 and Bax mRNA and protein expression levels. D The number of apoptotic cells was determined using annexin V/PI FC. Data represent mean ± SEM. **p < 0.01. PIAS3 protein inhibitor of activated STAT3, qPCR quantitative polymerase chain reaction, amyloid beta, WB western blotting, Bcl-2 B-cell lymphoma 2, Bax bcl-2-like protein 4, DMSO dimethyl sulfoxide, FC flow cytometry

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