Fig. 4

Effects of PIAS3 overexpression on oxidative stress in Aβ-treated SH-SY5Y cells. Cells were subjected to transfection with a PIAS3 overexpression vector or an empty vector for 2 d, and subsequently subjected to Aβ treatment (20 μM in 0.1% DMSO) for an additional 1.5 d. A, B ROS generation was determined by DCF-fluorescence and Amplex red stain at 24 h post transfection. C–F qPCR and WB were performed for determining the levels of NQO1, HMOX1, and GCLM in SH-SY5Y cells. Data represent mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001. PIAS3 protein inhibitor of activated STAT3, qPCR quantitative polymerase chain reaction, Aβ amyloid beta, WB western blotting, ROS reactive oxygen species, DMSO dimethyl sulfoxide