Fig. 5

Effects of PIAS3 overexpression on STAT3 activation and Nestin/Nrf2/HO-1 pathway in Aβ-treated SH-SY5Y cells. A–D qPCR was used to determine the mRNA level expressions of STAT3, Nestin, Nrf2, and HO-1. E WB was utilized for determining the protein level expressions of STAT3, Nestin, Nrf2, and HO-1, and STAT3 phosphorylation. F ARE luciferase activity was also determined. G Fluorescence microscopy was performed to detect the expression and location of STAT3, Nrf2, and Nestin in cells. Scale bar, 50 μm. *P < 0.05, **P < 0.01 vs. indicated groups. Data represent mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001. PIAS3 protein inhibitor of activated STAT3, STAT3 signal transducer and activator of transcription 3, Nrf2 nuclear factor erythroid 2-related factor, HO-1 heme oxygenase-1, qPCR quantitative polymerase chain reaction, Aβ amyloid beta, WB western blotting, ARE antioxidant responsive element