Fig. 7

Effects of colivelin treatment on cell survival, oxidative stress, and apoptosis in Aβ-treated and PIAS3-overexpressing SH-SY5Y cells. Cells were subjected to transfection with a PIAS3 overexpression vector or an empty vector for 1 d, and subsequently subjected to Aβ treatment (20 μM in 0.1% DMSO) and colivelin treatment (0.5 μM in 0.1% DMSO) for an additional 1 d. A, B ROS generation was determined by DCF-fluorescence and Amplex red stain at 24 h post transfection. C–F qPCR and WB were utilized for determining the expression levels of NQO1, HMOX1, and GCLM in SH-SY5Y cells. G–I qPCR and WB were used for determining Bcl-2 and Bax mRNA and protein expressions. J The number of apoptotic cells was determined using annexin V/PI FC. K The CCK-8 assay demonstrated the influence of PIAS3 on cell survival 2 d after treatment. L CFA showed the influence of PIAS3 upregulation on cell growth of SH-SY5Y cells. Data represent mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001. PIAS3 protein inhibitor of activated STAT3, qPCR quantitative polymerase chain reaction, Aβ amyloid beta, WB western blotting, ROS reactive oxygen species, Bcl-2 B-cell lymphoma 2, Bax bcl-2-like protein 4, CCK-8 Cell Counting Kit-8, CFA colony formation assay, FC flow cytometry, DMSO dimethyl sulfoxide