Fig. 1
IL-4 deficiency attenuates bone resorption in bone metastasis of CRC. A ELISA assay detected the protein level of IL-4 in bone marrow at each timepoint after intratibially injection of MC-38 cells (n = 4, each sample was pooled from 2 mice). B qRT-PCR analysis showed the mRNA level of IL-4 at each timepoint (n = 3–5, each sample was pooled from 2 mice). C Histochemistry analysis showed the bone resorption in tibias at 21 DPI in wild type mice and IL4KO mice (Scale bar = 100 μm) and quantification of trabecular area (n = 8). D μCT analysis compared the bone volume of tibias between wild type mice and IL4KO mice at 21 DPI (n = 3). E Quantification of bone mineral density (BMD). F Quantification of trabecular bone volume fraction (BV/TV). G–I Quantification of trabecular thickness (Tb.Th) (G), trabecular number (Tb. N) (H), trabecular separation (Tb. Sp) (I) (n = 3). J ELISA assay detected the protein level of IL-4 in bone marrow after intratibially injection of CT-26 cells at D0 and D10 (n = 5, each sample was pooled from 2 mice). K mRNA level of IL-4 in bone marrow derived from tibias after injection of CT-26 at D0 and D10 (n = 4, each sample was pooled from 2 mice). L Histochemistry analysis showed the bone resorption in tibias at 21 DPI after injection of MC-38 cells or CT-26 cells in vehicle treated or IL-4 nAb treated groups (Scale bar = 100 μm) and quantification of trabecular area (n = 9). *p < 0.05, **p < 0.01, ***p < 0.001