Fig. 2
Neutrophils-derived IL-4 stimulates the proliferation of early OCPs. A TRAP staining showed the TRAP( +) cells in trabecular area at D21 after injection of MC-38 in WT mice or IL4KO mice and the quantification of OC area in bone surface (Scale bar = 100 μm) (n = 9). B TRAP staining showed the TRAP( +) cells in trabecular area at D21 after injection of CT-26 after treatment with vehicle or IL-4 neutralizing antibody and the quantification of OC area in bone surface (Scale bar = 100 μm) (n = 9). C Flow cytometry plots and quantification of the percentage of early OCPs at each timepoint after injection of MC-38 cells into tibias and the quantification (n = 3–9 per condition, each sample was pooled from 2 mice). D Flow cytometry plots and quantification of the percentage of BrdU( +) early OCPs at each timepoint after injection of MC-38 cells into tibias and the quantification (n = 3–6, each sample was pooled from 2 mice). E Flow cytometry plots and quantification of the percentage of BrdU( +) early OCPs in WT mice and IL4KO mice at 10 days post injection of MC-38 cells. (n = 4, each sample was pooled from 2 mice). F Flow cytometry plots and quantification of the percentage of BrdU( +) early OCPs cultured in MC-38 CM after stimulated by vehicle or IL-4. (n = 3) G Flow cytometry plots and quantification of the percentage of BrdU( +) early OCPs cultured in MC-38 CM with or without IL-4 neutralizing antibody. (n = 6) H Flow cytometry plots of the percentage of IL-4 ( +) cells in neutrophils, macrophages or neutrophils, respectively. *p < 0.05, **p < 0.01