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Fig. 3 | Molecular Medicine

Fig. 3

From: IL4/IL4R signaling promotes the osteolysis in metastatic bone of CRC through regulating the proliferation of osteoclast precursors

Fig. 3

IL4Rα regulating the proliferation of early OCPs in metastatic bone of CRC. A Transcriptomic profiling revealing most changed pathways analyzed by KEGG pathway analysis (left) and scatter plot showing the differentially expressed genes (right). B qRT-PCR analysis detected the mRNA expression of IL4Rα in early OCPs cultured in MC-38 conditioned medium. (n = 3) C qRT-PCR analysis and western blots showing the mRNA level and protein level of IL4Rα or IL13rα in early OCPs isolated from bone marrow at each timepoint after injection of MC-38 cells. (n = 3–5 per condition, each sample was pooled from 3 mice) D Flow cytometry plots and quantification of the percentage of IL4Rα ( +) early OCPs isolated from bone marrow at each timepoint after injection of MC-38 cells. (n = 6–9 per condition) E Flow cytometry plots and quantification of percentage of BrdU( +) OCPs cultured in MC-38 CM after treated by recombinant IL-4 protein with/without transfection with IL4Rα-siRNA. (n = 3) F Flow cytometry plots and quantification of the percentage of IL4Rα ( +) early OCPs isolated from bone marrow at D0 and D10 after injection of CT-26 cells. (n = 3) *p < 0.05, **p < 0.01, ***p < 0.001

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