Fig. 1

MFAP5 expression was associated with osteogenesis. A MFAP5 expression was lower in the BMSCs of patients with osteoporosis compared to those with osteoarthritis. B Osteogenic induction of human mesenchymal stromal cells (hMSCs) at 0, 1, 2, 3, and 4 days showed that the level of mRNA encoding MFAP5 was positively associated with osteogenesis. C The expression patterns of MFAP5 in C3H10 and MC3T3-E1 cells during osteogenic differentiation, as revealed by Western blotting and the quantitative results of Western blotting. D Mineralized nodules and ALP expression revealed by AR-S and ALP staining during the osteogenesis of C3H10 and MC3T3-E1 cells. E The relative ALP expression levels of the two cell lines. F Quantitation of AR-S staining of C3H10 and MC3T3-E1 cells. G and H The levels of mRNAs encoding MFAP5, Runx2, Col1α1, and OCN of both cell types during osteogenesis. Values are expressed as means ± standard deviations (*P < 0.05, **P < 0.01, ***P < 0.001)