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Fig. 2 | Molecular Medicine

Fig. 2

From: Microfibrillar-associated protein 5 regulates osteogenic differentiation by modulating the Wnt/β-catenin and AMPK signaling pathways

Fig. 2

MFAP5 knockdown inhibited osteogenesis. A The knockdown efficiency of the three shRNAs; protein levels were quantified by Western blotting. B The mRNA level of MFAP5 in C3H10 and MC3T3-E1 cells, as revealed by qRT-PCR. C AR-S and ALP staining showed that MFAP5 knockdown inhibited the osteogenic capacity of C3H10 and MC3T3-E1 cells. D and E Quantitative analyses of ALP expression and AR-S staining. F–H The mRNA levels of classical biomarkers of osteogenic differentiation (Runx2, Col1α1, and OCN) in the shRNA and NC groups. Values are expressed as means ± standard deviations (*P < 0.05, **P < 0.01, ***P < 0.001)

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Molecular Medicine

ISSN: 1528-3658