Fig. 4From: Microfibrillar-associated protein 5 regulates osteogenic differentiation by modulating the Wnt/β-catenin and AMPK signaling pathwaysMFAP5 overexpression promoted osteogenesis, which was mediated by the Wnt/β-catenin and AMPK signaling pathways. A, B C3H10 cells overexpressed MFAP5 in protein and mRNA. C The C3H10-cDNA group had more calcified nodules and higher ALP expression than the other groups during osteogenic induction. D, E The ALP activity level and AR-S staining revealed that the osteogenic capacity of the two groups differed. F The MFAP5, β-catenin, p-GSK-3β, GSK-3β, AMPK, and p-AMPK expression levels indicated that the Wnt/β-catenin and AMPK signaling pathways were more highly activated in the C3H10-cDNA group. G–I Relative protein levels of β-catenin, p-GSK-3β/ GSK-3β and p-AMPK/AMPK. J–L The levels of the classical osteogenic biomarkers Runx2, Col1α1, and OCN in the C3H10-NC and C3H10-cDNA groups. Values are expressed as means ± standard deviations (*P < 0.05, **P < 0.01, ***P < 0.001)Back to article page