Fig. 3

Effect of HCG18 knockdown on the proinflammatory process and HCG18 targeting of miR-29a/b. A Effect of HCG18 knockdown on cytokines as determined by qRT–PCR. B HCG18 knockdown reduced NF-κB activity, as shown by luciferase assays. C, D Effect of HCG18 knockdown on NF-κB and AKT signalling pathways by Western blot. E, F HCG18 knockdown reduced TRAF4/5 expression in EOC cells, as determined by Western blot and qRT–PCR. G HCG18 knockdown increased miR-29a/b levels in EOC cells, as determined by qRT–PCR. n = 3. *P < 0.05, **P < 0.01, ***P < 0.001