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Fig. 6 | Molecular Medicine

Fig. 6

From: LncRNA HCG18 upregulates TRAF4/TRAF5 to facilitate proliferation, migration and EMT of epithelial ovarian cancer by targeting miR-29a/b

Fig. 6

Further analysis in OVSAHO cell lines. A, B The interaction of HCG18 and miR-29a/b, as well as of TRAF4/5 mRNA and miR-29a/b was determined by dual luciferase reporter assay in OVSAHO cells. OVSAHO cells were transfected with shHCG18, shHCG18 + TRAF4/5 plasmids, then the cell proliferative ability (C, D), cell migration capacity (E), the expression of EMT markers and transcription factors (F, G), and the levels of cytokines (H) were measured by MTT, colony formation, Transwell, Western blot and qRT-PCR, respectively. n = 3. *P < 0.05, **P < 0.01, ***P < 0.001

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Molecular Medicine

ISSN: 1528-3658