Fig. 1From: Metformin alleviates the calcification of aortic valve interstitial cells through activating the PI3K/AKT pathway in an AMPK dependent wayHistochemical staining of aortic valves. a and b Hematoxylin–eosin (HE) and Masson’s trichrome staining showing valve thickening and collagen fiber hyperplasia; c Von Kossa staining showing calcium deposition in valves, black indicates calcium deposition. Scale bar, 200 μm; CAVD calcific aortic valve diseaseBack to article page