Fig. 6
From: Rab31-dependent regulation of transforming growth factor ß expression in breast cancer cells
Western blot analysis of TGF-ß1 in cell culture supernatants of Rab31 low- and high-expressing cells. Concentrated cell culture supernatants (concentration factor 1:16; the total protein content of the supernatants was used for normalization) of CAMA-1 (A) or MDA-MB231 (B) Rab31 overexpressing and vector control cells, respectively, were reacted with a TGF-ß1 specific antibody. The signals obtained match the expected size for pro-TGF-ß1 and its cleavage product LAP, respectively