Fig. 8

Validation of differential gene expression in Rab31 overexpressing and vector control cells. A Validation of the significant, more than twofold, down-regulation of the five genes TGFB1, TGFB2, FSTL3, BMP7, and SMAD6 in Rab31 overexpressing and vector control cells. B Quantitative determination of the B2M, FAS, and TNFSF10 mRNA levels CAMA-1 cells. The significant, more than twofold up-regulation of the genes B2M and TNFSF10 in Rab31 overexpressing and vector control cells is in line with the profiler PCR array analyses (see Table 2). In case of FAS, although significant, only an approx. 1.4-fold increase is observed in Rab31 overexpressing over vector control cells. In all cases, except for TNFSF10, the mRNA levels from vector control cells were set to 100% and the relative mRNA levels in the corresponding Rab31 overexpressing cells was calculated accordingly. For TNFSF10 expression, the mRNA levels of Rab31 overexpressing cells were set to 100%. All samples were normalized to HPRT1 expression level. Three experiments were performed in triplicates each. Statistically significant differences (p < 0.05) are indicated by an asterisk