Table 2 Summary of main findings
Analysis type | Subject | Identity | Working hypothesis | Experimental clues | References |
|---|---|---|---|---|---|
Individual CK2 sites | Viral proteins | N | CK2 phosphosite inhibition/blockage by CIGB-300 impairs viral replication | Interaction, co-localization, N mRNA and protein expression inhibition by CIGB-300 in a subrogate model | Ramon et al. (2021) |
Individual CK2 sites | Viral proteins | Orf6 | CK2 phosphosite inhibition/blockage by CIGB-300 restores IFN signaling | Additive/Synergistic profile of CIGB-300 plus IFN alpha | Unpublished |
Co-Expression and network propagation | Host and Viral Proteins | B23, Orf6, NUP98 | CK2 phosphosite inhibition/blockage by CIGB-300 restores IFN signaling | Additive/Synergistic profile of CIGB-300 plus IFN alpha | Unpublished |
Phosphoproteome overlap | Host Proteins | MATR3, SQSTM1, DIDO1 | CIGB-300 Multitarget effect impairing viral transcription/splicing, inflammation, immunoresponse and apoptosis | Pulmonary lesions resolution in CT Phase I | Cruz et al. (2020) |
Enrichment analysis at early viral infection and Kinase activity profiles | Host proteins | SRPK1 (SRSF1 and SRSF-2,6,10) | CIGB-300 impairs SRSF1 role in viral protein synthesis | None, to be evaluated in preclinical settings | NA |
PPIs vs H125 Phosphoproteome | Host and Viral Proteins | Several proteins (see text and Fig. 7) [LARP1, LARP7, LARP4B] | CIGB-300 impairs Viral RNA processing and transcription | None, to be evaluated in preclinical settings | NA |
SARS-CoV-2 vs H125 Phosphoproteome overlap, Network and Enrichment analysis | Host proteins | Several proteins (see text and Fig. 8) [HNRNPA1, HSPB1, SRRM2, SRRM1, B23] | CIGB-300 Multitarget effect impairing on mRNA metabolism, cell cycle and Autophagy pathways | Pulmonary lesions resolution in CT Phase I | Cruz et al. (2020) |
Enrichment of Human Phenotypes | Host proteins | HNPRNPA1, MATR3, SQSTM1, B23, FIP1L1, NUMA1 | CIGB-300 may relief Covid-19 clinical symptoms | To be evaluated in CT Phase II | NA |