Fig. 2

Omega-3 FAs ameliorate LPS-induced intestinal epithelial cell injury by downregulating miR-1-3p. A The expression of miR-1-3p in LPS-induced sepsis cell model as determined by RT-qPCR. B The transfection efficiency of miR-1-3p mimic as determined by RT-qPCR. C The expression of miR-1-3p in LPS-induced cells in response to miR-1-3p mimic or/and omega-3 FAs as determined by RT-qPCR. D The levels of TNF-α, IL-1β and IL-6 in the supernatant of LPS-induced cells in response to miR-1-3p mimic or/and omega-3 FAs as determined by ELISA. E The levels of ROS and MDA and the activity of SOD in LPS-induced cells in response to miR-1-3p mimic or/and omega-3 FAs as detected by ROS, MDA and SOD detection kits. F The apoptosis of LPS-induced cells in response to miR-1-3p mimic or/and omega-3 FAs as examined by flow cytometry. G The expression of cleaved-caspase-3 and caspase-3 in LPS-induced cells in response to miR-1-3p mimic or/and omega-3 FAs as detected by Western blot assay. *p < 0.05. Measurement data were expressed as mean ± standard deviation. Unpaired t-test was performed for the data analysis between two groups while one-way ANOVA with Tukey's test for data analysis among multiple groups. Experiment was repeated independently three times