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Fig. 4 | Molecular Medicine

Fig. 4

From: Omega-3 fatty acids impair miR-1-3p-dependent Notch3 down-regulation and alleviate sepsis-induced intestinal injury

Fig. 4

Notch3 inhibits the Smad signaling and attenuates LPS-induced intestinal epithelial cell injury. A The activation of Smad in the sepsis cell model as measured by Western blot assay. B The activation of Notch3 and Smad in LPS-induced cells treated with oe-Notch3 or oe-Notch3 + activin as measured by Western blot assay. C The levels of TNF-α, IL-1β and IL-6 in supernatant of LPS-induced cells treated with oe-Notch3 or oe-Notch3 + activin as determined by ELISA. D Determination of ROS and MDA levels and SOD activity in LPS-induced cells treated with oe-Notch3 or oe-Notch3 + activin. E The apoptosis of LPS-induced cells treated with oe-Notch3 or oe-Notch3 + activin as examined by TUNEL staining. F The expression of cleaved-caspase-3 and caspase-3 in LPS-induced cells treated with oe-Notch3 or oe-Notch3 + activin as determined by Western blot assay. *p < 0.05. Measurement data were expressed as mean ± standard deviation. Unpaired t-test was performed for the data analysis between two groups while one-way ANOVA with Tukey's test for data analysis among multiple groups. Experiment was repeated independently three times

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