Fig. 5

Omega-3 FAs mediate the miR-1-3p/Notch3/Smad axis to attenuate LPS-induced intestinal epithelial cell injury. A The expression of miR-1-3p as determined by RT-qPCR and the activation of Notch3 and Smad in LPS-induced cells treated with omega-3 FAs or omega-3 FAs + activin as measured by Western blot assay. B The levels of TNF-α, IL-1β and IL-6 in supernatant of LPS-induced cells treated with omega-3 FAs or omega-3 FAs + activin as determined by ELISA. C Determination of ROS and MDA levels and SOD activity in LPS-induced cells treated with omega-3 FAs or omega-3 FAs + activin. D The apoptosis of LPS-induced cells treated with omega-3 FAs or omega-3 FAs + activin as examined by TUNEL staining. E The expression of cleaved-caspase-3 and caspase-3 in LPS-induced cells treated with omega-3 FAs or omega-3 FAs + activin as determined by Western blot assay. *p < 0.05. Measurement data were expressed as mean ± standard deviation. Unpaired t-test was performed for the data analysis between two groups. Experiment was repeated independently three times