Fig. 6

Omega-3 FAs mediate the miR-1-3p/Notch3/Smad signal axis to alleviate sepsis-induced intestinal injury. A The expression of miR-1-3p and the activation of Notch3 and Smad in intestinal tissues of mice with different treatment as measured by RT-qPCR and Western blot assay. B Body weight of mice with different treatment within 3 days after sepsis induction. C Survival rate of mice with different treatment within 3 days after sepsis induction. D Bacterial content in peritoneal lavage fluid and blood of mice with different treatment. E The expression of TNF-α, IL-1β and IL-6 in serum of mice with different treatment as determined by ELISA. F Determination of ROS and MDA levels and SOD activity in mice with different treatment. G Pathological changes of intestinal tissues of mice with different treatment as observed by HE staining. H The pathological changes of colonic mucosa as observed by TEM. I The apoptosis of intestinal tissues as examined by TUNEL staining. J The expression of cleaved-caspase-3 and caspase-3 as determined by immunohistochemistry. n = 8. *p < 0.05. Measurement data were expressed as mean ± standard deviation. Unpaired t-test was performed for the data analysis between two groups while one-way ANOVA with Tukey's test for data analysis among multiple groups, and repeated measures ANOVA with Tukey's test for data analysis at different time points. Experiment was repeated independently three times