Fig. 4
Synergistic effects of ISL1 and KDM6B on SNAI1. A Expressions of KDM6B and SNAI1 in liver tissues of mice exposed to high-fat diet detected by Western blot analysis. B The correlation between ISL1 and SNAI1 in mouse liver tissues was analyzed by Pearson correlation analysis. C The correlation between KDM6B and SNAI1 in mouse liver tissues was analyzed by Pearson correlation analysis. D Expressions of KDM6B and SNAI1 in an in vitro model of lipid accumulation determined by Western blot analysis. E RT-qPCR was used to detect the expression of ISL1/KDM6B/SNAI1 in THLE-2 cells after overexpressing or knockdown of ISL1/KDM6B. F Western blot was used to detect the expression of ISL1/KDM6B/SNAI1 in THLE-2 cells after overexpressing/knockdown of ISL1/KDM6B. G Co-IP detection of the binding between ISL1 and KDM6B in vitro. H Immunofluorescence detection of the localization of ISL1 and KDM6B in cells. I Oil red O staining shows steatosis in response to artificial modulation of ISL1 and KDM6B expression. J ChIP detection of effect of overexpressing/knockdown of KDM6B on KDM6B enrichment and methylation degree in SNAI1 promoter region. *p < 0.05, ***p < 0.001 vs. NC treatment; ###p < 0.001 vs. oe-ISL1 treatment. Cell experiment was repeated 3 times independently. n = 8 mice in each group