Fig. 6

Synergistic alleviative effects of ISL1 and KDM6B on NAFLD by upregulating the expression of SNAI1. A RT-qPCR was used to detect the effect of overexpressing ISL1/KDM6B and knockdown of SNAI1 on the expression levels of ISL1, KDM6B, and SNAI1 in the liver tissues of mice exposed to high-fat diet. B Western blot analysis was applied to detect the effect of overexpressing ISL1/KDM6B and knockdown of SNAI1 on the expression levels of ISL1, KDM6B, and SNAI1 in the liver tissues of mice exposed to high-fat diet. C Oil red O staining was applied to detect the effect of overexpressing ISL1/KDM6B and knockdown of SNAI1 on liver lipid accumulation in mouse model. D Effect of overexpressing ISL1/KDM6B and knockdown of SNAI1 on the plasma contents of TG and TC in mice exposed to high-fat diet. E Effect of overexpressing ISL1/KDM6B and knockdown of SNAI1 on the contents of TG and TC in liver tissues of mice exposed to high-fat diet. F Western blot analysis was conducted to detect the effect of overexpressing ISL1/KDM6B and knockdown of SNAI1 on lipid synthesis- and lipolysis-related genes in liver tissues of mice exposed to high-fat diet. G Oil red O staining was performed to detect the effect of overexpressing ISL1/KDM6B and knockdown of SNAI1 on lipid accumulation. H Western blot analysis was used to detect the effect of overexpressing ISL1/KDM6B and knockdown of SNAI1 on the expressions of ISL1, KDM6B, SNAI1, lipid synthesis- and lipolysis-related genes in the in vitro model of lipid accumulation. ***p < 0.001 vs. oe-NC + sh-NC treatment; ^###p < 0.001 vs. oe-ISL1 + sh-NC treatment; ^&&&p < 0.001 vs. oe-KDM6B + sh-NC treatment. Cell experiment was repeated 3 times independently. n = 8 mice in each group