Fig. 3

β-HB decreased PES1 and triglycerides in cultured liver cells. a, b The protein levels of CHOP, PES1, p300, SREBP1c, SREBP2, FASN and SCD1 in hepatocytes were detected by immunoblotting after 1 mM β-HB treatment for 24 h. c β-HB decreased CHOP binding to Pes1 promoter. d Exhibited are immunofluorescence images of β-HB-treated liver cells for PES1 and SREBP1c expression and localization, Scale bar represents 20 um. The nuclei were stained with DAPI. e, f Demonstrated are the TG and TC levels respectively detected in the cultured media and liver cells after β-HB treatment. Ctrl (Control), β-HB (β-hydroxybutyric acid). Data were shown as mean ± SEM for each experiment performed independently 3 times. *P < 0.05, **P < 0.01, ***P < 0.001 compared with control (Student’s t test)