Fig. 4

Effects of Pes1 knockdown and overexpression on lipid metabolism in vitro. a, b Pes1 knockdown in cultured liver cells inhibited the protein levels of PES1, p300, SREBP1c, FASN and SCD1. c, d Displayed are the effects of Pes1 knockdown on the TG and TC levels in cultured liver cells and media. e, f Pes1 overexpression enhanced PES1-Flag, SREBP1c, p300, FASN and SCD1 protein levels in cultured liver cells, while β-HB treatment abolished the effects. g, h Exhibited are the effects of Pes1 overexpression on the TG and TC levels in cultured liver cells and media. Data were shown as mean ± SEM for each experiment performed independently 3 times. *P < 0.05, **P < 0.01, ***P < 0.001 compared with controls (Student’s t-test or ANOVA, Student–Newman–Keuls q test)