Fig. 1
From: Characterisation of cold-induced mitochondrial fission in porcine aortic endothelial cells
Cold-induced mitochondrial fission and its reversibility. Mitochondria of porcine aortic endothelial cells stained with MitoTracker Red (A–C) are shown before cold incubation (A), after cold incubation at 4 °C for 48 h (B) and after rewarming for 1 h at 37 °C (C). Mitochondrial membrane potential (TMRM staining, red) is shown before cold incubation (D), after cold incubation for 48 h (E) and after rewarming (F) in mitochondria counter-stained with MitoTracker Green (green). Cell viability was assessed by propidium iodide uptake and Hoechst 33342 counter-staining (G–I), its quantification (J) and assessment of LDH release (J). Caspase 3 was analysed as an apoptosis marker; cycloheximide (Chx; 50 µg/ml) served as positive control (K). Representative figures of n = 4 experiments; for J means ± SD of n = 4 experiments. (MitoTracker Red/TMRM/PI: λexc. = 546 ± 6 nm, λem. ≥ 590 nm; MitoTracker Green: λexc. = 470 ± 20 nm, λem. = 525 ± 25 nm; Hoechst: λexc. = 359 ± 24 nm, λem. = 445 ± 25 nm)