Fig. 10From: Characterisation of cold-induced mitochondrial fission in porcine aortic endothelial cellsCellular ATP content during cold incubation and rewarming. A Aortic endothelial cells (control, ctrl, open bar) were exposed to 4 °C for 1 h, 3 h, 6 h, 24 h and 48 h (grey bars) and rewarmed for 1 h at 37 °C (hatched bars; e.g. 48/1). B Additionally, ATP content was measured after 48 h at 4 °C and rewarming for 1 h at 37 °C with and without the tubulin polymerization inhibitor nocodazole (Noco; added 18 h before rewarming). ATP was measured in cell lysates with a bioluminescence assay kit. Means ± SD of n = 4 experiments. Images of the microtubuli (green; C–G) and mitochondria (red; H–L) are shown (MitoTracker Red: λexc. = 546 ± 6 nm, λem. ≥ 590 nm; FITC: λexc. = 470 ± 20 nm, λem. = 525 ± 25 nm). Representative figures of n = 4 experiments. * Significant difference to the control (p ≤ 0.05)Back to article page