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Fig. 2 | Molecular Medicine

Fig. 2

From: Characterisation of cold-induced mitochondrial fission in porcine aortic endothelial cells

Fig. 2

Time dependence of cold-induced mitochondrial fission. Cultured porcine aortic endothelial cells (A) were exposed to hypothermia for 3 h (B), 6 h (C), 12 h (D), 24 h (E) and 48 h (F). Mitochondria were stained by MitoTracker Red and documented using fluorescence microscopy (λexc. = 546 ± 6 nm, λem. ≥ 590 nm). Representative figures of n = 4 experiments. For quantification (G) cells, incubated at 4 °C for the indicated time (i.e. 3 h) and rewarmed for 1 h at 37 °C (e.g. 3/1), were categorised in cells with predominantly short, intermediate or long mitochondria by a person blinded to the experiment. Warm/rewarming conditions are shown hatched. Means ± SD of n = 4 experiments. *Significant difference of long mitochondria compared to control (p ≤ 0.05)

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