Fig. 6
From: Characterisation of cold-induced mitochondrial fission in porcine aortic endothelial cells
Qualitative and quantitative analysis of proteins involved in mitochondrial fission and outer membrane fusion. Protein lysates of porcine aortic endothelial cells incubated for 3 h, 6 h, 24 h or 48 h at 4 °C and rewarmed for 1 h at 37 °C were analysed by western blot (A) for protein levels of Drp1 and phosphorylation of Drp1 (activating site p-Drp1-S616 and inactivating site p-Drp1-S637) and Fis1. Additionally Mff, p-Mff Ser146, MiD49, MiD51, Mfn1 and Mfn2 were targeted. Coomassie staining was used as loading control (coom.). Representative figures of n = 4 experiments. The band intensity was quantified using the BioVision software and normalized to the control (B–D). Rewarming conditions are shown hatched. Means ± SD of n = 4 experiments. * Significantly different to control (p ≤ 0.05) *** (p ≤ 0.005) # Significantly different to the respective cold incubation (p ≤ 0.05)