Table 2 Partial purification of ADA from rat joint tissues
From: Quercetin mitigates rheumatoid arthritis by inhibiting adenosine deaminase in rats
Steps | Volume (ml) | Total protein (mg) | Total activity (mU) | Specific activity (mU/mg) | Purification fold | Recovery % |
|---|---|---|---|---|---|---|
Crude homogenate | 10 | 16,080 | 94.3 | 0.0058 | 1 | 100 |
High speed supernatant | 9.3 | 2848 | 43.65 | 0.015 | 2.58 | 46.2 |
20% Ammonium sulfate | 4.2 | 737.26 | 17.947 | 0.025 | 4.31 | 18.98 |
Dialysis | 3.2 | 18.42 | 14.95 | 0.81 | 137.28 | 15.85 |
Gel filtration Sephadex (G-100) | 8.2 | 2.08 | 12.3 | 5.913 | 996 | 13 |