Fig. 7

Western blot of nuclear β-catenin and cytoplasmic myofibroblast markers of HUVEC with increased barometric pressure. Pressurized or nonpressurized HUVECs were treated with control solvent DMSO or pyrvinium pamoate to create the four experimental groups. A Western blots of nuclear protein extracts for β-catenin. Lamin B1 served as the loading control. B Quantification of nuclear β-catenin. C Western blots of cytoplasmic protein extracts for αSMA and ITGB6. GAPDH served as the loading control. D Quantification of cytoplasmic αSMA. E Quantification of cytoplasmic ITGB6. Data are presented as median, 1st and 3rd quartiles, maximal and minimal values in the box plots (n = 5 per group). P values in B, D, and E were determined by the Wilcoxon signed-rank test with Bonferroni correction. Insignificant P values are not shown. HUVEC human umbilical endothelial venous cell, DMSO dimethyl sulfoxide, αSMA smooth muscle alpha-actin, ITGB6 integrin subunit β6, GAPDH glyceraldehyde-3-phosphate dehydrogenase