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Fig. 3 | Molecular Medicine

Fig. 3

From: When left does not seem right: epigenetic and bioelectric differences between left- and right-sided breast cancer

Fig. 3

L/R Ca2+ concentration comparison. MDA-MB-231 cells treated with L/R normal breast extracts from donor W1 and measurement of intracellular calcium concentration with Fluo3 AM. A Representative calcium fluorescence in two-dimensional (a and b) and three-dimensional images-surface plot- (a′ and b′). Pseudo-color from black to red represents low to high intracellular calcium concentration, respectively. B Fluorescence quantification was performed on still images (×20 magnification), by calculating the fluorescence mean of 50–100 cells fields. The analysis reveals a significant increment of intracellular calcium concentration in the L-treated cells. Values represent the mean ± SEM from 3 independent experiments, 50–100 cells were analyzed per experiment (unpaired T-test with Welch’s correction, p < 0.003). A.U artificial units

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