Fig. 5

Lysine acetylation and PCAF expression is increased and ROS levels is elevated in aortic-VSMC from GK rats in advanced T2DM. Aortic VSMCs were isolated from Wistar and GK rats and maintained in primary cultures. a Morphology of aortic VSMCs cultures in passage 1. b Global lysine acetylation was detected by using a specific antibody for acetylated lysine residue. β actin used as internal control. c Representative immunoblotting for PCAF (top panel). β tubulin used as internal control. d ROS levels detected by DHE fluorescence in each experimental group. Quantification of DHE staining was determined through fluorescent intensity in each cell by pixel intensity of the cell. Bar graphs are means ± SEM of four independent experiments as determined from densitometry relative to internal control. *p < 0.05 vs. control