Fig. 7

Garcinol reduces HG-induced ROS production in hVSMC. Quiescent hVSMC were pretreated with 15 µM garcinol, followed by stimulation with 25 mM HG or 5 mM NG for 60 min. a ROS production was detected using DHE staining. Representative photomicrographs depicting DHE fluorescence in each experimental group. Quantification of DHE staining was determined through fluorescent intensity in each cell by pixel intensity of the cell. b Global lysine acetylation was detected by western blot. β actin used as internal control. Results represent means ± SEM. n = 4 per group, *p < 0.05 vs. controls, **p < 0.05 vs. HG group